mouse tubular epithelial cells mtecs  (ATCC)

Journal: International Journal of Medical Sciences

Article Title: Upregulation of C/EBP Homologous Protein induced by ER Stress Mediates Epithelial to Myofibroblast Transformation in ADTKD-UMOD

doi: 10.7150/ijms.65036

Figure Lengend Snippet: CHOP knockdown restores the upregulation of vimentin and FN induced by TM. HK-2 (A,C) and mTEC (B,D) were transfected with siRNA against CHOP (siCHOP1 and siCHOP2) or control siRNA for 24 h, then treated with TM (HK2 in 10 nM and mTEC in 20nM for 48 h). Representative western blots and quantification of the relative protein levels are presented (A,B). Gene expression analysis of vimentin and fibronectin in the HK2 (C) and mTEC (D) were analyzed after treated with TM for 24 h. At least three independent experiments were performed. # P < 0.05, * P < 0.05, NS, no significant.

Article Snippet: For in vitro experiments, human renal tubular epithelial cells (HK2) and mouse renal tubular epithelial cells (mTEC) (acquired from ATCC, American Tissue Culture Collection) were cultured in DMEM/F12 containing 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a 5% CO 2 /95% air atmosphere.

Techniques: Knockdown, Transfection, Control, Western Blot, Gene Expression